Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid)procedure.

نویسندگان

  • A A Dietz
  • H M Rubinstein
  • T Lubrano
چکیده

Submitters: Albert A. Dietz, Herbert M. Rubinstein, and Tina Lubrano, Research and Medical Services, VA Hospital, Hines, III. 60141; and Departments of Biochemistry and Medicine, Loyola University Stritch School of Medicine, Maywood, III. 60153 Evaluators: Philip J. Garry, Department of Pediatrics, Children’s Hospital, Ohio State University, Columbus, Ohio 43205 Frank Madera-Orsini, Biochemistry Department, West Suburban Hospital, Oak Park, III. 60302 Bill C. Strever, Department of Clinical Pathology and Biochemistry, Medical University of South Carolina, Charleston, S. C. 29401

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منابع مشابه

Expressing lower limits of normal.

1512 CLINICAL CHEMISTRY, Vol. 25, No. 8, 1979 brano, T., Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid) procedure. Selected Methods Clin. Chem. 8,41-46 (1977). 5. King, J., Studies on human serum cholinesterases. Ph.D. thesis, University of Glasgow, 1974. 6. Garry, P. J., Serum cholinesterase variants: Examina...

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Effect of daylight on the reaction of thiols with Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid).

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A Micro Method for Serum Cholinesterase.

A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetyithiocholine to release thiocholine, which reacts with 5:5-dithiobis(2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 mm. at 370; the reaction is stopped by the excess addition of quinidine sulfate. The difference in absor...

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Comparison of a commercially available assay system with two reference methods for the determination of plasma cholinesterase variants.

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عنوان ژورنال:
  • Clinical chemistry

دوره 19 11  شماره 

صفحات  -

تاریخ انتشار 1973