Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid)procedure.
نویسندگان
چکیده
Submitters: Albert A. Dietz, Herbert M. Rubinstein, and Tina Lubrano, Research and Medical Services, VA Hospital, Hines, III. 60141; and Departments of Biochemistry and Medicine, Loyola University Stritch School of Medicine, Maywood, III. 60153 Evaluators: Philip J. Garry, Department of Pediatrics, Children’s Hospital, Ohio State University, Columbus, Ohio 43205 Frank Madera-Orsini, Biochemistry Department, West Suburban Hospital, Oak Park, III. 60302 Bill C. Strever, Department of Clinical Pathology and Biochemistry, Medical University of South Carolina, Charleston, S. C. 29401
منابع مشابه
Expressing lower limits of normal.
1512 CLINICAL CHEMISTRY, Vol. 25, No. 8, 1979 brano, T., Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid) procedure. Selected Methods Clin. Chem. 8,41-46 (1977). 5. King, J., Studies on human serum cholinesterases. Ph.D. thesis, University of Glasgow, 1974. 6. Garry, P. J., Serum cholinesterase variants: Examina...
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The reaction of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman's reagent) with thiols is sensitive to daylight, in particular to ultraviolet radiation at wavelengths around 325 nm. Exposure to light at the absorbance maximum of the yellow product (the thionitrobenzoate ion) at 410 nm had no effect on the reaction. The light-sensitive species is apparently the DTNB, because a spectral-irradia...
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A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetyithiocholine to release thiocholine, which reacts with 5:5-dithiobis(2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 mm. at 370; the reaction is stopped by the excess addition of quinidine sulfate. The difference in absor...
متن کاملComparison of a commercially available assay system with two reference methods for the determination of plasma cholinesterase variants.
For assaying plasma cholinesterase (EC 3.1.1.8) activity and phenotyping by means of dibucaine inhibition, we have compared a commercially available kit, in which butyrylthiocholine is used as substrate, with two reference methods, one using benzoylcholine and the other propionylthiocholine. With 50 different samples of three of the most common genetic variants, we could clearly differentiate t...
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1. A rapid colorimetric and apparently specific micromethod for the determination of total glutathione in small amounts of tissue is described. Generally, less than 30mg. of tissue is sufficient and this is homogenized in ice-cold 3% metaphosphoric acid. The product is filtered through sintered glass and neutralized or diluted before being added to a cuvette containing phosphate buffer, pH7.1, ...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 11 شماره
صفحات -
تاریخ انتشار 1973